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M9480867.TXT
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1994-09-05
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Document 0867
DOCN M9480867
TI Analysis of sewage effluent for human immunodeficiency virus (HIV) using
infectivity assay and reverse-transcriptase polymerase chain reaction.
DT 9410
AU Palmer CJ; Lee MH; Bonilla GF; Javier BJ; Tsai YL; Sangermano LR; Orange
County Sanitation Districts, Fountain Valley, CA.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:415 (abstract no. Q-154).
Unique Identifier : AIDSLINE ASM94/94313078
AB Environmental survival of human immunodeficiency virus (HIV) is an
important public health concern. Survival of HIV in wastewater is of
particular interest to those who work at treatment facilities and to the
general public who have contact with rivers or ocean water receiving
treated sewage effluent. Other researchers have reported that HIV can be
detected in wastewater. These studies, however, detected homologous
nucleic acid sequences but did not determine infectivity. This current
study tested for the presence of HIV in primary and secondary sewage
from a major metropolitan sewage agency using both reverse-transcriptase
polymerase chain reaction (RT-PCR) and infectivity testing. For RT-PCR,
primers SK38 and SK39 (from the HIV1 gag region) were used to amplify
HIV fragments from concentrated and extracted sewage samples.
Infectivity assays were completed on peripheral blood mononuclear cells
(PBMC) obtained from a blood donor stimulated with phytohaemagglutinin.
For infectivity assays, sewage was concentrated and neutralized with
human immunoglobulin (free of HIV antibody) to eliminate other viruses
normally present in sewage. In addition, coxsackie B4, echo 7, and polio
1, viruses normally prevalent in sewage, were tested to determine their
effect on the PBMC line. Interestingly, polio 1 was found to infect the
PBMC line. This was an unexpected result. Testing of the treated sewage
for the presence of HIV, however, showed that PCR and infectivity assays
were negative for the presence of HIV in both primary and secondary
treated sewage. HIV seeded into sewage remained infectious as detected
by the PBMC coculture procedure used in this study, therefore, HIV
replication may not be inhibited in sewage samples.
DE DNA Primers Genes, gag Human HIV/GROWTH & DEVELOPMENT/*ISOLATION &
PURIF/PATHOGENICITY Lymphocytes/MICROBIOLOGY Polymerase Chain
Reaction/*METHODS *Sewage Urban Health Virulence Viruses/GROWTH &
DEVELOPMENT/ISOLATION & PURIF MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).